Article
Structure and mechanism of GDP-mannose glycosyl hydrolase, a Nudix enzyme that cleaves at carbon instead of phosphorus.
Structure (London, England : 1993) - 1 Jun 2004
Gabelli Sandra B, Bianchet Mario A, Azurmendi Hugo F, Xia Zuyong, Sarawat Vibhor, Mildvan Albert S, Amzel L Mario
Abstract excerpt
GDP-mannose glycosyl hydrolase (GDPMH) catalyzes the hydrolysis of GDP-mannose and GDP-glucose to GDP and sugar by substitution with inversion at C1 of the sugar. The enzyme has a modified Nudix motif and requires one divalent cation for activity. The 1.3 A X-ray structure of the GDPMH-Mg(2+)-GDP complex, together with kinetic, mutational, and NMR data, suggests a mechanism for the GDPMH reaction. Several...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.