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Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction.

Biochemistry - 28 Jun 2005

Xia Zuyong, Azurmendi Hugo F, Lairson Luke L, Withers Stephen G, Gabelli Sandra B, Bianchet Mario A, Amzel L Mario, Mildvan Albert S

Abstract excerpt

GDP-mannose hydrolase (GDPMH) catalyzes the hydrolysis of GDP-alpha-d-sugars by nucleophilic substitution with inversion at the anomeric C1 atom of the sugar, with general base catalysis by H124. Three lines of evidence indicate a mechanism with dissociative character. First, in the 1.3 A X-ray structure of the GDPMH-Mg(2+)-GDP.Tris(+) complex [Gabelli, S. B., et al. (2004) Structure 12, 927-935], the GDP leaving...

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PMID: 15966723
DOI: 10.1021/bi050583v

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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