Article
Lipopolysaccharide heterogeneity in Escherichia coli J5 variants: analysis by flow cytometry.
The Journal of infectious diseases - 1 Oct 1992
Evans M E, Pollack M, Koles N L, Hardegen N J, Panopoulos D
Abstract excerpt
A lipopolysaccharide O-side chain-producing phenotypic variant was isolated from a uridine 5'-diphosphogalactose 4-epimeraseless Escherichia coli J5 rough mutant strain by fluorescence-activated cell sorting with a monoclonal antibody (MAb) specific for the O-side chain of E. coli O111:B4 smooth parent lipopolysaccharide. The variant (J5-2) was recognized by both core- and O-side chain-specific MAbs, while the...
Topics
- Antibodies, Monoclonal
- Cell Separation
- Escherichia coli
- Flow Cytometry
- Immunoblotting
- Lipopolysaccharides
- Phenotype