Article
The importance of methionine residues for the catalysis of the biotin enzyme, transcarboxylase. Analysis by site-directed mutagenesis.
The Journal of biological chemistry - 15 Sept 1992
Shenoy B C, Xie Y, Park V L, Kumar G K, Beegen H, Wood H G, Samols D
Abstract excerpt
Almost all biotin enzymes contain the conserved tetrapeptide Ala-Met-Bct-Met (Bct, N epsilon-biotinyl-L-lysine). In the 1.3 S biotinyl subunit of transcarboxylase (TC), this sequence is present between positions 87 and 90. The conserved nature of these amino acids implies a critical role in the f...
Topics
- Amino Acid Sequence
- Base Sequence
- Carboxyl and Carbamoyl Transferases
- Catalysis
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Kinetics
- Methionine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation