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Article

Mutation of exposed hydrophobic amino acids to arginine to increase protein stability.

BMC biochemistry - 13 Jul 2004

Strub Caroline, Alies Carole, Lougarre Andrée, Ladurantie Caroline, Czaplicki Jerzy, Fournier Didier

Abstract excerpt

BACKGROUND: One strategy to increase the stability of proteins is to reduce the area of water-accessible hydrophobic surface. RESULTS: In order to test it, we replaced 14 solvent-exposed hydrophobic residues of acetylcholinesterase by arginine. The stabilities of the resulting proteins were tested using denaturation by high temperature, organic solvents, urea and by proteolytic digestion. CONCLUSION: Although the...

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Identifiers

PMID: 15251041
DOI: 10.1186/1471-2091-5-9
PMCID: PMC479692

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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