Article
A new method for detecting single nucleotide polymorphism using GFP-display.
Journal of biochemical and biophysical methods - 30 Jul 2004
Aoki Takashi, Satoh Keiko, Imamura Tomoko, Watabe Hiroyuki
Abstract excerpt
The single nucleotide polymorphism (SNP) of aldehyde dehydrogenase-2 (ALDH2) codon 487, GAA (Glu) or AAA (Lys), was examined using green fluorescent protein (GFP)-display, an electrophoretic detection method for single amino acid changes. Although no shift in migration between the GFP-ALDH (Glu487) and GFP-ALDH (Lys487) fusion proteins was observed on SDS/urea gel, the two migrated to different positions when...
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