Article
Neurogenesis in hippocampal slice cultures.
Molecular and cellular neurosciences - 1 Jun 2004
Raineteau Olivier, Rietschin Lotty, Gradwohl Gérard, Guillemot François, Gähwiler Beat H
Abstract excerpt
A major challenge in studying neurogenesis in the adult brain is gaining access to neural stem cells for experimental manipulation. We developed an approach utilizing mouse hippocampal organotypic cultures to characterize neurogenesis under controlled conditions. After 2 weeks in culture, double immunostaining using the mitotic marker BrdU and cell type-specific markers revealed persistent proliferation of...
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