Article
Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis.
The Journal of biological chemistry - 5 Aug 1994
Ochsner U A, Fiechter A, Reiser J
Abstract excerpt
Transposon Tn5-GM-induced mutant strains of Pseudomonas aeruginosa which are unable to produce rhamnolipid biosurfactants and lack rhamnosyltransferase activity have been isolated. The DNA regions flanking the transposon were cloned and used as specific probes for the isolation of the correspondi...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Carbohydrate Sequence
- DNA, Bacterial
- Decanoates
- Disaccharides
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Hexosyltransferases
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Pseudomonas aeruginosa
- Rhamnose
- Surface-Active Agents