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Article

In vivo selection for the directed evolution of L-rhamnulose aldolase from L-rhamnulose-1-phosphate aldolase (RhaD).

Bioorganic & medicinal chemistry - 1 Sept 2007

Sugiyama Masakazu, Hong Zhangyong, Greenberg William A, Wong Chi-Huey

Abstract excerpt

Dihydroxyacetone phosphate (DHAP)-dependent aldolases have been widely used for organic synthesis. The major drawback of DHAP-dependent aldolases is their strict donor substrate specificity toward DHAP, which is expensive and unstable. Here we report the development of an in vivo selection system for the directed evolution of the DHAP-dependent aldolase, L-rhamnulose-1-phosphate aldolase (RhaD), to alter its...

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Identifiers

PMID: 17572092
DOI: 10.1016/j.bmc.2007.05.062
PMCID: PMC1992742

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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