Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Effects of N-terminal deletion mutation on rabbit muscle lactate dehydrogenase.

Biochemistry. Biokhimiia - 1 Apr 2004

Zheng Yanbin, Guo Shuyuan, Guo Zhi, Wang Xicheng

Abstract excerpt

Deletion mutants of rabbit muscle lactate dehydrogenase (LDH) were constructed using polymerase chain reaction (PCR) to study the roles of N-terminal residues. The coding sequences of the first 5 (LD5) and 10 (LD10) amino acids of the N-terminus were deleted and the gene was inserted into the prokaryotic expression vector pET21b. The mutant enzymes were expressed in E. coli BL21/DE3 and were purified. Then their...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Enzyme Stability
Share to Muscles
Share to Protein Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Protein Denaturation

Share to Rabbits

Share to Recombinant Proteins

Share to Sequence Deletion

Share to Structure-Activity Relationship

Source

Identifiers

PMID: 15170375
DOI: 10.1023/b:biry.0000026195.31821.e1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Effects of N-terminal deletion mutation on rabbit muscle lactate dehydrogenase.PMID 15170375Read ↗

Role of the linker between the N- and C-terminal domains in the stability and folding of rabbit muscle creatine kinase.Semantic proximity Read ↗

Biochemical and in silico Characterization of Recombinant L-Lactate Dehydrogenase of Theileria annulata.Semantic proximity Read ↗

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of L-lactate dehydrogenase and its H171C mutant from Bacillus subtilis.Semantic proximity Read ↗

Characterization of the Pro101Gln mutation that enhances the catalytic performance of T. indicus NADH-dependent d-lactate dehydrogenase.Semantic proximity Read ↗

Mutations that significantly change the stability, flexibility and quaternary structure of the l-lactate dehydrogenase from Bacillus megaterium.Semantic proximity Read ↗

Distinct conformation-mediated functions of an active site loop in the catalytic reactions of NAD-dependent D-lactate dehydrogenase and formate dehydrogenase.Semantic proximity Read ↗

Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain.Semantic proximity Read ↗

The flexibility of the non-conservative region at the C terminus of D-hydantoinase from Pseudomonas putida YZ-26 is extremely limited.Semantic proximity Read ↗

Single mutation in Shine-Dalgarno-like sequence present in the amino terminal of lactate dehydrogenase of Plasmodium effects the production of an eukaryotic protein expressed in a prokaryotic system.Semantic proximity Read ↗

Effects of N-terminal deletion mutation on arginine kinase from the sea cucumber Stichopus japonicus.Semantic proximity Read ↗

Effect of foreign N-terminal residues on the conformational stability of human lysozyme.Semantic proximity Read ↗

Engineering of Aerococcus viridans L-lactate oxidase for site-specific PEGylation: characterization and selective bioorthogonal modification of a S218C mutant.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.