Article
Mutations that significantly change the stability, flexibility and quaternary structure of the l-lactate dehydrogenase from Bacillus megaterium.
European journal of biochemistry - 15 Jan 1993
Kotik M, Zuber H
Abstract excerpt
In order to investigate the physical basis of protein stability, two mutant L-lactate dehydrogenases (LDH) and the wild-type enzyme from Bacillus megaterium were analyzed for differences in quaternary structure, global protein conformation, thermal stability, stability against guanidine hydrochlo...
Topics
- Bacillus megaterium
- Circular Dichroism
- Fructosephosphates
- Guanidines
- Hot Temperature
- Kinetics
- L-Lactate Dehydrogenase
- Models, Molecular
- Motion
- Mutation
- NAD
- Protein Binding
- Protein Conformation
- Protein Denaturation
- Recombinant Proteins
- Spectrometry, Fluorescence
- Structure-Activity Relationship