Article
E-cadherin-coated plates maintain pluripotent ES cells without colony formation.
PloS one - 20 Dec 2006
Nagaoka Masato, Koshimizu Uichi, Yuasa Shinsuke, Hattori Fumiyuki, Chen Hao, Tanaka Tomofumi, Okabe Masaru, Fukuda Keiichi, Akaike Toshihiro
Abstract excerpt
Embryonic stem (ES) cells cultured on gelatin-coated plates or feeder layers form tight aggregated colonies by the E-cadherin-mediated cell-cell adhesions. Here we show that murine ES cells do not make cell-cell contacts or form colonies when cultured on the plate coated with a fusion protein of...
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