Article
Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs.
Genome research - 1 Jun 2004
Chevreux Bastien, Pfisterer Thomas, Drescher Bernd, Driesel Albert J, Müller Werner E G, Wetter Thomas, Suhai Sándor
Abstract excerpt
We present an EST sequence assembler that specializes in reconstruction of pristine mRNA transcripts, while at the same time detecting and classifying single nucleotide polymorphisms (SNPs) occuring in different variations thereof. The assembler uses iterative multipass strategies centered on high-confidence regions within sequences and has a fallback strategy for using low-confidence regions when needed. It...
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