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Article

[Significantly improvement of antibody expression level in CHO cells through downregulation of the DHFR gene in expression vector].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Jan 2003

Bai Yin, Wang Yan, Zhang Hai-rong, Zhou Li-jun, Lu Ying-qian, Yu Li-zhang

Abstract excerpt

AIM: To increase the expressed level of a human-mouse chimeric antibody against human bladder tumor in dihydrofolate reductase (DHFR) defective CHO cells(CHO/DHFR) via weakening the transcription of DHFR gene in the vector. METHODS: A series of chimeric antibody expression vectors with different deletions and mutations in the modulator sequence of DHFR gene were constructed to downregulate the DHFR gene...

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Source

Identifiers

PMID: 15132910

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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