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Article

Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells.

BMC biotechnology - 2 Jun 2015

Chin Christine Lin, Chin Hing Kah, Chin Cara Sze Hui, Lai Ethan Tingfeng, Ng Say Kong

Abstract excerpt

BACKGROUND: Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity. Destabilization of dihydrofolate reductase (dhfr) selection marker by addition of AU-rich elements and murine ornithine decarboxylase PEST region was previously shown to improve the specific productivities of...

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Identifiers

PMID: 26033090
DOI: 10.1186/s12896-015-0145-9
PMCID: PMC4450478

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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