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Rat cytochrome P450C24 (CYP24A1) and the role of F249 in substrate binding and catalytic activity.

Archives of biochemistry and biophysics - 15 May 2004

Annalora Andrew, Bobrovnikova-Marjon Ekaterina, Serda Rita, Lansing Letitia, Chiu Mark L, Pastuszyn Andrzej, Iyer Srinivas, Marcus Craig B, Omdahl John L

Abstract excerpt

A high level of functional recombinant rat cytochrome P450C24 enzyme (CYP24A1) was obtained (40-50mg/L) using an Escherichia coli expression system. Purified enzyme was stable with retention of spectral and catalytic activity. The rate of 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] side-chain oxidation and cleavage to the end-product calcitroic acid was directly related to the rate of electron transfer from the...

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Identifiers

PMID: 15111121
DOI: 10.1016/j.abb.2004.01.025

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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