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Article

In vivo and in vitro function of GroEL mutants with impaired allosteric properties.

The Journal of biological chemistry - 1 Dec 2000

Fridmann Y, Ulitzur S, Horovitz A

Abstract excerpt

Escherichia coli cells that produce only plasmid-encoded wild-type or mutant GroEL were generated by bacteriophage P1 transduction. Effects of mutations that affect the allosteric properties of GroEL were characterized in vivo. Cells containing only GroEL(R197A), which has reduced intra-ring positive cooperativity and inter-ring negative cooperativity in ATP binding, grow poorly upon a temperature shift from 25...

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Identifiers

PMID: 10973985
DOI: 10.1074/jbc.M007594200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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