Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue.

The Journal of biological chemistry - 2 Jul 2004

Lairson Luke L, Chiu Cecilia P C, Ly Hoa D, He Shouming, Wakarchuk Warren W, Strynadka Natalie C J, Withers Stephen G

Abstract excerpt

Lipopolysaccharyl-alpha-1,4-galactosyltransferase C (LgtC), a glycosyltransferase family 8 alpha-1,4-galactosyltransferase from Neisseria meningitidis, catalyzes the transfer of galactose from UDP galactose to terminal lactose-containing acceptor sugars with net retention of anomeric configuration. To investigate the potential role of discrete nucleophilic catalysis suggested by the double displacement mechanism...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Aspartic Acid
Share to Chromatography, Liquid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Galactose

Share to Glycosyltransferases

Share to Lactose

Share to Models, Chemical

Share to Neisseria meningitidis

Share to Spectrometry, Mass, Electrospray Ionization

Source

Identifiers

PMID: 15075344
DOI: 10.1074/jbc.M400451200

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue.PMID 15075344Read ↗

Retaining glycosyltransferase mechanism studied by QM/MM methods: lipopolysaccharyl-α-1,4-galactosyltransferase C transfers α-galactose via an oxocarbenium ion-like transition state.Semantic proximity Read ↗

Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs.Semantic proximity Read ↗

Biochemical and structural insights into an allelic variant causing the lysosomal storage disorder - aspartylglucosaminuria.Semantic proximity Read ↗

Structural and kinetic analysis of Bacillus subtilis N-acetylglucosaminidase reveals a unique Asp-His dyad mechanism.Semantic proximity Read ↗

A transitional hydrolase to glycosynthase mutant by Glu to Asp substitution at the catalytic nucleophile in a retaining glycosidase.Semantic proximity Read ↗

Theoretical study of enzymatic catalysis explains why the trapped covalent intermediate in the E303C mutant of glycosyltransferase GTB was not detected in the wild-type enzyme.Semantic proximity Read ↗

Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: molecular insights and effector modulation of N-acetylglucosaminyltransferase I.Semantic proximity Read ↗

A catalytically essential motif in external loop 5 of the bacterial oligosaccharyltransferase PglB.Semantic proximity Read ↗

Mechanism-based inhibitors of glycosidases: design and applications.Semantic proximity Read ↗

Bioconjugation and detection of lactosamine moiety using alpha1,3-galactosyltransferase mutants that transfer C2-modified galactose with a chemical handle.Semantic proximity Read ↗

tRNA-guanine transglycosylase from Escherichia coli: molecular mechanism and role of aspartate 89.Semantic proximity Read ↗

Engineering of Helicobacter pylori L-asparaginase: characterization of two functionally distinct groups of mutants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.