Article
Autoregulation of the yeast lysyl-tRNA synthetase gene GCD5/KRS1 by translational and transcriptional control mechanisms.
Cell - 21 Aug 1992
Lanker S, Bushman J L, Hinnebusch A G, Trachsel H, Mueller P P
Abstract excerpt
We cloned the GCD5 gene of S. cerevisiae and found it to be identical to KRS1, which encodes lysyl-tRNA synthetase (LysRS). The mutation gcd5-1 changes a conserved residue in the putative lysine-binding domain of LysRS. This leads to a defect in lysine binding and, consequently, to reduced charging of tRNA(Lys). Mutant gcd5-1 cells compensate for the defect in LysRS by increasing GCN4 expression at the...
Topics
- Alleles
- Amino Acid Sequence
- DNA Mutational Analysis
- DNA-Binding Proteins
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Lysine-tRNA Ligase
- Molecular Sequence Data
- Protein Biosynthesis
- Protein Kinases