Simultaneous quantification and genotyping of hepatitis C virus RNA by a two-step real-time PCR assay on the lightcycler instrument.

doi:10.2478/v10153-010-0003-4

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Article

Simultaneous quantification and genotyping of hepatitis C virus RNA by a two-step real-time PCR assay on the lightcycler instrument.

Folia medica - 1 Jan 2000

Abe Manabu, Klett Corinne, Wieland Eberhard, Gille Sascha, Landt Olfert

Abstract excerpt

BACKGROUND: Clinically, both viral load and genotypes have been found to be major predictors of antiviral therapy outcome regarding chronic hepatitis C and they are, under normal circumstances, performed as separate assays. DESIGN AND METHODS: In order to improve the diagnostic strategy and subsequently reduce the reagent costs we have developed and established the simultaneous quantification and genotyping of...

Topics

Base Sequence
Genotype
Hepacivirus
Humans
Molecular Sequence Data
RNA, Viral
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Transition Temperature
Viral Load

Source

Open in PubMed

Identifiers

PMID: 21053670
DOI: 10.2478/v10153-010-0003-4

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