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Article

Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures

29 Jan 2004

Abstract excerpt

BACKGROUND: The clinical efficacy of the current methods used for cryopreservation of metaphase II human oocytes is low. Meiotic spindle disorders are thought to be largely responsible for this situation. METHODS: Supernumerary fresh metaphase II human oocytes were cryopreserved in 1,2-propanediol with 0.1 M sucrose using a slow freezing/rapid thawing programme. Meiotic spindles were analysed in these living...

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PMID: 14998966
DOI: 10.1093/humrep/deh101

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