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Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration

11 Mar 2005

Abstract excerpt

BACKGROUND: One of the major concerns derived from the cryopreservation of meiotically mature oocytes is possible damage to the cytoskeletal apparatus, and in particular the meiotic spindle. METHODS: One hundred fresh oocytes showing the polar body I and high meiotic spindle birefringence (maximum retardance+/-1.5 mol/l SD = 2.58+/-0.1 nm), assessed through analysis, were included in this study. Oocytes were...

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PMID: 15760952
DOI: 10.1093/humrep/deh736

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