Article
A new sequenced allelic ladder marker for D1S80 typing.
Journal of human genetics - 1 Jan 2004
Fujii Koji, Sekiguchi Kazumasa, Shimizu Kenshi, Kasai Kentaro
Abstract excerpt
A sequenced allelic ladder marker that contains 32 alleles consisting of alleles 13-44 was developed for D1S80 ( MCT118) typing. Each allele, after amplification with D1S80 primers, was cloned to the pT7Blue T-Vector. The plasmid DNAs of these clones were mixed and amplified with D1S80 primers to construct the allelic ladder marker. All the alleles prepared in this study were sequenced in both directions, using...
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