Article
Multigenerational amplification of a reference ladder for alleles at locus D1S80.
Journal of forensic sciences - 1 Sept 1993
Baechtel F S, Smerick J B, Presley K W, Budowle B
Abstract excerpt
The interlaboratory typing of DNA specimens that have been amplified at the D1S80 locus necessitates the use of a standard allelic reference ladder. This communication describes a technique in which individual, amplified alleles are isolated, combined, and amplified by PCR to produce a functional reference ladder composed of many of the alleles that occur at this locus. The amplified ladder can serve directly as...
Topics
- Alleles
- Chromosome Mapping
- DNA Fingerprinting
- Gene Amplification
- Humans
- Polymerase Chain Reaction
- Reference Values
- Repetitive Sequences, Nucleic Acid
