Article
Nucleotide substitution in the 5' flanking region of D1S80 locus.
Forensic science international - 19 Sept 1997
Watanabe G, Umetsu K, Yuasa I, Vogt U, Suzuki T
Abstract excerpt
We have analyzed a mutation responsible for a Hinf I polymorphism of the D1S80 system by Taq dye deoxy terminator cycle sequencing. A G-T transversion at the 58th nucleotide from the 5' end of the forward primer was identified. This mutation has been firmly established by the restriction enzymes. The type G was digested with Hinf I but not with Tsp 509 I. Conversely, the type T was digested with Tsp 509 I but not...
Topics
- Base Sequence
- DNA Fingerprinting
- DNA Mutational Analysis
- DNA-Directed DNA Polymerase
- Deoxyribonucleases, Type II Site-Specific
- Gene Frequency
- Germany
- Humans
- Japan
- Minisatellite Repeats
- Mutation
- Polymorphism, Restriction Fragment Length
- Reproducibility of Results
- Taq Polymerase
- Terminator Regions, Genetic