Article
[Study on the construction of standard D1S549 allelic ladder via molecular cloning and its genetic polymorphism in Chinese three populations].
Fa yi xue za zhi - 1 Aug 2001
Zhang L, Xin J P, Chen G D, Tian X, Liao M, Li R H
Abstract excerpt
OBJECTIVE: To resolve the problem of the accuracy and standardization of STR-PCR typing in forensic practice, we have designed a new method to produce standard D1S549 allelic ladder. METHODS: Eight different PCR amplified D1S549 allelic fragments were isolated from the gel, eluted into the distilled water and re-amplified by PCR. The purified allelic fragments were then blunt-end subcloned individually into the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.