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Article

Simple and rapid bioluminescent detection of two verotoxin genes using allele-specific PCR of E. coli O157: H7.

Luminescence : the journal of biological and chemical luminescence - 1 Jan 2000

Imamura O, Arakawa H, Maeda M

Abstract excerpt

Allele-specific PCR for E. coli O157 was conducted with primers specific to verotoxin genes, verotoxin 1 (VT1) and verotoxin 2 (VT2). VT is an important cause of haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS) worldwide. We developed a simple, rapid bioluminescent detection method for E. coli O157. The method is based on the determination of pyrophosphoric acid (PPi) released during...

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Identifiers

PMID: 12687631
DOI: 10.1002/bio.437

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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