Article
Detection of single-nucleotide polymorphism on uidA gene of Escherichia coli by a multiplexed electrochemical DNA biosensor with oligonucleotide-incorporated nonfouling surface.
Sensors (Basel, Switzerland) - 1 Jan 2011
Liu Gang, Lao Ruojun, Xu Li, Xu Qin, Li Lanying, Zhang Min, Shen Hao, Mathur Sanjay, Fan Chunhai, Song Shiping
Abstract excerpt
We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on...
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