Article
Mutational analysis of different regions in the coxsackievirus 2B protein: requirements for homo-multimerization, membrane permeabilization, subcellular localization, and virus replication.
The Journal of biological chemistry - 7 May 2004
de Jong Arjan S, Melchers Willem J G, Glaudemans Dirk H R F, Willems Peter H G M, van Kuppeveld Frank J M
Abstract excerpt
The coxsackievirus 2B protein is a small hydrophobic protein (99 amino acids) that increases host cell membrane permeability, possibly by forming homo-multimers that build membrane-integral pores. Previously, we defined the functional role of the two hydrophobic regions HR1 and HR2. Here, we investigated the importance of regions outside HR1 and HR2 for multimerization, increasing membrane permeability,...
Topics
- Amino Acid Sequence
- Animals
- Arginine
- Asparagine
- Aspartic Acid
- COS Cells
- Cell Line
- Cell Membrane
- Chlorocebus aethiops
- DNA Mutational Analysis
- Dimerization
- Enterovirus
- Glutamic Acid