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Article

Analysis of the 3' cis-acting elements of rubella virus by using replicons expressing a puromycin resistance gene.

Journal of virology - 1 Mar 2004

Chen Min-Hsin, Frolov Ilya, Icenogle Joseph, Frey Teryl K

Abstract excerpt

A rubella virus (RUB) replicon, RUBrep/PAC, was constructed and used to map the 3' cis-acting elements (3' CSE) of the RUB genome required for RUB replication. The RUBrep/PAC replicon had the structural protein open reading frame partially replaced by a puromycin acetyltransferase (PAC) gene. Cells transfected with RUBrep/PAC transcripts expressed the PAC gene from the subgenomic RNA, were rendered resistant to...

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Share to Acetyltransferases
Share to Chlorocebus aethiops
Share to Gene Expression Regulation, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Puromycin

Share to Regulatory Sequences, Ribonucleic Acid

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Source

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Identifiers

PMID: 14963158
DOI: 10.1128/jvi.78.5.2553-2561.2004
PMCID: PMC369209

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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