Article
Determination of a specific region of the purine-cytosine permease involved in the recognition of its substrates.
Molecular microbiology - 1 Oct 1992
Bloch J C, Sychrova H, Souciet J L, Jund R, Chevallier M R
Abstract excerpt
Three u.v.-induced mutants of the purine-cytosine permease gene of Saccharomyces cerevisiae, with altered apparent Michaelis constant of transport (Kmapp), were cloned and sequenced. One of the mutants had extensive nucleotide replacement, whereas the other two had a single mutation. To evaluate the contribution of the different amino acid replacements to the phenotype of the complex mutant, simpler mutants were...
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