Article
Replacement of Lys by Glu in a transmembrane segment strongly impairs the function of the uracil permease from Saccharomyces cerevisiae.
The Biochemical journal - 15 Jun 1995
Urban-Grimal D, Pinson B, Chevallier J, Haguenauer-Tsapis R
Abstract excerpt
The co-transport of uracil and protons through the plasma membrane of the yeast Saccharomyces cerevisiae is mediated by a specific permease encoded by the FUR4 gene. The uracil permease is a multi-spanning membrane protein that follows the secretory pathway to the plasma membrane. Recent experime...
Topics
- Blotting, Western
- Carrier Proteins
- Cell Membrane
- Glutamic Acid
- Kinetics
- Lysine
- Membrane Proteins
- Membrane Transport Proteins
- Models, Molecular
- Mutation
- Nucleotide Transport Proteins
- Protein Binding
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transformation, Genetic
- Uracil