Article
A high-affinity uptake system for branched-chain amino acids in Saccharomyces cerevisiae.
Yeast (Chichester, England) - 1 Dec 1991
Tullin S, Gjermansen C, Kielland-Brandt M C
Abstract excerpt
In order to isolate mutants with impaired uptake of branched-chain amino acids, mutants were induced which on complex medium were sensitive to an inhibitor of branched-chain amino acid biosynthesis. Eighteen mutants of independent origin were found. Ten of them were assayed for branched-chain amino acid uptake. Three of these were impaired in the uptake of L-valine, L-isoleucine and L-leucine, while the rest were...
Topics
- Amino Acids, Branched-Chain
- Biological Transport
- Culture Media
- Isoleucine
- Kinetics
- Leucine
- Mutation
- Saccharomyces cerevisiae
- Valine
