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Article

A library of siRNA duplexes targeting the phosphoinositide 3-kinase pathway: determinants of gene silencing for use in cell-based screens.

Nucleic acids research - 1 Jan 2004

Hsieh Andrew C, Bo Ronghai, Manola Judith, Vazquez Francisca, Bare Olivia, Khvorova Anastasia, Scaringe Stephen, Sellers William R

Abstract excerpt

Gene silencing through RNA interference (RNAi) has been established as a means of conducting reverse genetic studies. In order to better understand the determinants of short interfering RNA (siRNA) knockdown for use in high-throughput cell-based screens, 148 siRNA duplexes targeting 30 genes within the PI3K pathway were selected and synthesized. The extent of RNA knockdown was measured for 22 genes by...

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Source

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Identifiers

PMID: 14769947
DOI: 10.1093/nar/gkh238
PMCID: PMC373385

Genes

PDK1
PTEN

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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