Electrochemical monitoring of transport by a vesicular monoamine transporter expressed in Xenopus oocytes.

doi:10.1016/j.jneumeth.2003.10.010

Article

Electrochemical monitoring of transport by a vesicular monoamine transporter expressed in Xenopus oocytes.

Journal of neuroscience methods - 15 Feb 2004

Whitley Janet, Parsons Jessica, Freeman Jeffrey, Liu Yongjian, Edwards Robert H, Near Joseph A

Abstract excerpt

Xenopus laevis oocytes were injected with synthetic mRNA coding for a rat VMAT2 mutant (rVMAT2-I483A/L484A) shown previously to be retained on the plasma membrane as a result of a presumed reduction of endocytosis. Binding of the specific VMAT inhibitor [3H]dihydrotetrabenazine indicated that expression did occur at a level of approximately 3 fmol per oocyte. To determine if rVMAT2-I483A/L484A expressed in...

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Electrochemical monitoring of transport by a vesicular monoamine transporter expressed in Xenopus oocytes.

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