Article
Structural requirements for the processing of Escherichia coli 5 S ribosomal RNA by RNase E in vitro.
Journal of molecular biology - 20 Dec 1992
Cormack R S, Mackie G A
Abstract excerpt
Processing of 9 S precursor RNA in Escherichia coli requires the endoribonuclease RNase E, which makes two cleavages to liberate p5, the immature form of 5 S rRNA. The contributions of primary and secondary structure to RNase E-mediated cleavage of 9 S RNA were investigated. The structure of the 5' domain of 9 S RNA was probed by partial ribonuclease digestion and chemical modification. Our structural analysis of...
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