Article
Maturation of precursor 10Sa RNA in Escherichia coli is a two-step process: the first reaction is catalyzed by RNase III in presence of Mn2+.
Biochimie - 1 Nov 1990
Srivastava R K, Miczak A, Apirion D
Abstract excerpt
A precursor to 10Sa RNA accumulates in an rne mutant. However, the present studies indicate that RNase III is the enzyme that processes this RNA. Cell extracts prepared from an rne mutant failed to cleave p10Sa RNA, whereas E coli wild type, rne and rnp cell extracts processed p10Sa RNA under specific assay conditions that require the presence of Mn2+ but not under the customary conditions used for assaying RNase...
Topics
- Endoribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Kinetics
- Magnesium
- Manganese
- Molecular Weight
- Mutation
- RNA Precursors
- RNA Processing, Post-Transcriptional
- RNA, Bacterial