Article
Multiplex PCR amplimer conformation analysis for rapid detection of gyrA mutations in fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates.
Antimicrobial agents and chemotherapy - 1 Feb 2004
Cheng Augustine F B, Yew Wing W, Chan Edward W C, Chin Miu L, Hui Mamie M M, Chan Raphael C Y
Abstract excerpt
A new strategy known as multiplex PCR amplimer conformation was developed for detection of mutation in the gyrA gene of 138 clinical isolates of Mycobacterium tuberculosis. The method generated a single-stranded and heteroduplex DNA banding pattern of multiplex PCR amplimers of the region of interest that was extremely sensitive to specific mutations, thus enabling much more sensitive and reliable mutation...
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