Article
Mapping cooperative activity of the hepatitis C virus RNA-dependent RNA polymerase using genotype 1a-1b chimeras.
Biochemical and biophysical research communications - 9 Jan 2004
Gu Baohua, Gutshall Lester L, Maley Derrick, Pruss Cynthia M, Nguyen Tammy T, Silverman Carol L, Lin-Goerke Juili, Khandekar Sanjay, Liu Changbao, Baker Audrey E, Casper David J, Sarisky Robert T
Abstract excerpt
The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) encodes an RNA-dependent RNA polymerase (RdRp) which is essential for viral replication. NS5B expression in bacteria generated 20- to 50-fold lower yield and 100-fold less product per mol of enzyme for gentoype 1a RdRp than type 1b. Further, unlike type 1b RdRp, type 1a enzyme failed to exhibit cooperative properties in the assays described herein....
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