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Article

Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling.

The EMBO journal - 1 Dec 1992

Leberer E, Dignard D, Hougan L, Thomas D Y, Whiteway M

Abstract excerpt

The STE4 gene, which encodes the beta subunit of the mating response G-protein in the yeast Saccharomyces cerevisiae, was subjected to a saturation mutagenesis using 'doped' oligodeoxynucleotides. We employed a genetic screen to select dominant-negative STE4 mutants, which when overexpressed from the GAL1 promoter, interfered with the signalling function of the wild type protein. The identified inhibitory amino...

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Identifiers

PMID: 1464310
DOI: 10.1002/j.1460-2075.1992.tb05586.x
PMCID: PMC556956

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling.PMID 1464310Read ↗

The Leu-132 of the Ste4(Gbeta) subunit is essential for proper coupling of the G protein with the Ste2 alpha factor receptor during the mating pheromone response in yeast.Semantic proximity Read ↗

The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components.Semantic proximity Read ↗

Dominant-negative mutations in the G-protein-coupled alpha-factor receptor map to the extracellular ends of the transmembrane segments.Semantic proximity Read ↗

Genetic interactions indicate a role for Mdg1p and the SH3 domain protein Bem1p in linking the G-protein mediated yeast pheromone signalling pathway to regulators of cell polarity.Semantic proximity Read ↗

Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1.Semantic proximity Read ↗

Biochemical and genetic analysis of dominant-negative mutations affecting a yeast G-protein gamma subunit.Semantic proximity Read ↗

Ste50p sustains mating pheromone-induced signal transduction in the yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

N-terminal intrinsic disorder is an ancestral feature of Gγ subunits that influences the balance between different Gβγ signaling axes in yeast.Semantic proximity Read ↗

Evolution of a G protein-coupled receptor response by mutations in regulatory network interactions.Semantic proximity Read ↗

Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway.Semantic proximity Read ↗

Regulation of the yeast pheromone response pathway by G protein subunits.Semantic proximity Read ↗

Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein.Semantic proximity Read ↗

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