Article
Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling.
The EMBO journal - 1 Dec 1992
Leberer E, Dignard D, Hougan L, Thomas D Y, Whiteway M
Abstract excerpt
The STE4 gene, which encodes the beta subunit of the mating response G-protein in the yeast Saccharomyces cerevisiae, was subjected to a saturation mutagenesis using 'doped' oligodeoxynucleotides. We employed a genetic screen to select dominant-negative STE4 mutants, which when overexpressed from the GAL1 promoter, interfered with the signalling function of the wild type protein. The identified inhibitory amino...
Topics
- Amino Acid Sequence
- GTP-Binding Protein beta Subunits
- GTP-Binding Proteins
- Genes, Dominant
- Heterotrimeric GTP-Binding Proteins
- Molecular Sequence Data
- Mutation
- Pheromones
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins