Article
The hepatitis C virus 5' untranslated region gene amplified by rapid amplification of cDNA ends and its secondary structure.
Hepatobiliary & pancreatic diseases international : HBPD INT - 1 Aug 2002
Qin Zhao-Xi, Cong Xu, Jiang Dong, Ha Ming-Hao, Chen Hong-Song, Wei Lai
Abstract excerpt
OBJECTIVES: To obtain very end full-length cDNA of hepatitis C virus (HCV) 5'untranslated region (5'UTR) and analyze its primary and secondary structure. METHODS: A patient infected genotype 2a HCV was identified by reverse transcription-nested polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). Total RNA isolated from the serum was used as template, and the cDNA of the...
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