Article
Effect of mutant small, acid-soluble spore proteins containing cysteine or tryptophan on DNA properties in vivo and in vitro.
Biochimie - 1 Jan 2000
Sanchez-Salas J L, Sharon M, Setlow P
Abstract excerpt
Two derivatives of the alpha/beta-type small acid-soluble spore protein (SASP) SspCwt have been constructed, each containing a residue potentially useful for physico-chemical analysis of protein-protein or protein-DNA interactions. In one mutant protein (SspCtrp) residue 27 (Met) was replaced by...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Cysteine
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Mutation
- Polydeoxyribonucleotides
- Pyrimidine Dimers
- Sigma Factor
- Spectrometry, Fluorescence
- Spores, Bacterial
- Transcription Factors
- Tryptophan