Article
Acquisition of photosynthetic capacity by a reaction centre that lacks the Q(A) ubiquinone; possible insights into the evolution of reaction centres?
Biochimica et biophysica acta - 17 Oct 2003
Wakeham Marion C, Frolov Dmitrij, Fyfe Paul K, van Grondelle Rienk, Jones Michael R
Abstract excerpt
A photosynthetically impaired strain of Rhodobacter sphaeroides containing reaction centres with an alanine to tryptophan mutation at residue 260 of the M-polypeptide (AM260W) was incubated under photosynthetic growth conditions. This incubation produced photosynthetically competent strains containing suppressor mutations that changed residue M260 to glycine or cysteine. Spectroscopic analysis demonstrated that...
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