Article
The effect of exchange of bacteriopheophytin a with plant pheophytin a on charge separation in Y(M210)W mutant reaction centers of Rhodobacter sphaeroides at low temperature.
Biochimica et biophysica acta - 6 Mar 2003
Shkuropatov Anatoli Ya, Neerken Sieglinde, Permentier Hjalmar P, de Wijn Rik, Schmidt Kristiane A, Shuvalov Vladimir A, Aartsma Thijs J, Gast Peter, Hoff Arnold J
Abstract excerpt
The bacteriopheophytin a molecules at the H(A) and H(B) binding sites of reaction centers (RCs) of the Y(M210)W mutant of Rhodobacter sphaeroides were chemically exchanged with plant pheophytin a. The Y(M210)W mutation slows down the formation of H(A)(-), presumably by raising the free energy level of the P(+)B(A)(-) state above that of P* due to increasing the oxidation potential of the primary electron donor P...
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