Article
Selective perturbation of the second electron transfer step in mutant bacterial reaction centers.
Biochimica et biophysica acta - 22 Apr 2002
Schenkl Selma, Spörlein Sebastian, Müh Frank, Witt Heike, Lubitz Wolfgang, Zinth Wolfgang, Wachtveitl Josef
Abstract excerpt
In order to specifically perturb the primary electron acceptor B(A) -- a monomeric bacteriochlorophyll (BChl) a -- involved in bacterial photosynthetic charge separation (CS), the protein environment of B(A) in the reaction center (RC) of Rhodobacter sphaeroides was modified by site-directed mutagenesis. Isolated RCs were characterized by redox titrations, low temperature optical spectroscopy, ENDOR/TRIPLE...
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