Article
A novel mechanism of enhanced transcription activity and fidelity for influenza A viral RNA-dependent RNA polymerase.
Nucleic acids research - 7 Sept 2021
Xu Xinzhou, Zhang Lu, Chu Julie Tung Sem, Wang Yuqing, Chin Alex Wing Hong, Chong Tin Hang, Dai Zixi, Poon Leo Lit Man, Cheung Peter Pak-Hang, Huang Xuhui
Abstract excerpt
During RNA elongation, the influenza A viral (IAV) RNA-dependent RNA polymerase (RdRp) residues in the active site interact with the triphosphate moiety of nucleoside triphosphate (NTP) for catalysis. The molecular mechanisms by which they control the rate and fidelity of NTP incorporation remain elusive. Here, we demonstrated through enzymology, virology and computational approaches that the R239 and K235 in the...
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