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Study of 18S rRNA and rDNA stability by real-time RT-PCR in heat-inactivated<i>Cryptosporidium parvum</i>oocysts

1 Sept 2003

Abstract excerpt

The public health problem posed by Cryptosporidium parvum has led the water supply industry to develop analytical tools for detecting viable oocysts in water. In this study, we report on a TaqMan real-time reverse transcription-polymerase chain reaction (RT-PCR) method that targets and quantifies C. parvum 18S rRNA. To study the suitability of 18S rRNA as an indicator of Cryptosporidium oocyst viability, the...

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PMID: 14553917
DOI: 10.1016/s0378-1097(03)00538-x

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