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Detection ofToxoplasma gondiiOocysts in Water Sample Concentrates by Real-Time PCR

11 Apr 2009

Abstract excerpt

PCR techniques in combination with conventional parasite concentration procedures have potential for the sensitive and specific detection of Toxoplasma gondii oocysts in water. Three real-time PCR assays based on the B1 gene and a 529-bp repetitive element were analyzed for the detection of T. gondii tachyzoites and oocysts. Lower sensitivity and specificity were obtained with the B1 gene-based PCR than with the...

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PMID: 19363083
DOI: 10.1128/aem.00285-09

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