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Article

Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis.

Journal of clinical microbiology - 1 Sept 2002

Tanriverdi Sultan, Tanyeli Atila, Başlamişli Fikri, Köksal Fatih, Kilinç Yurdanur, Feng Xiaochuan, Batzer Glenda, Tzipori Saul, Widmer Giovanni

Abstract excerpt

Several real-time PCR procedures for the detection and genotyping of oocysts of Cryptosporidium parvum were evaluated. A 40-cycle amplification of a 157-bp fragment from the C. parvum beta-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYBR Green I melting curve analysis was used to confirm the specificity of the method when DNA extracted from fecal...

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Identifiers

PMID: 12202559
DOI: 10.1128/JCM.40.9.3237-3244.2002
PMCID: PMC130769

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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