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Detection of viable Giardia cysts by amplification of heat shock-induced mRNA

1 Jan 1997

Abstract excerpt

Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia. The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product. Since the presence of the product did not indicate whether the cysts were viable, these amplifications are a presence/absence test only. In...

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PMID: 8979360
DOI: 10.1128/aem.63.1.324-328.1997

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